Rapid and sensitive detection of SARS-CoV-2 antibodies by biolayer interferometry.

ABSTRACT: Serological testing to evaluate antigen-specific antibodies in plasma is generally performed by rapid lateral flow test strips that lack quantitative results or by high complexity immunoassays that are time- and labor-intensive but provide semi-quantitative results. Here, we describe a novel application of biolayer interferometry for the rapid detection of antigen-specific antibody levels in plasma samples, and demonstrate its utility for quantification of SARS-CoV-2 antibodies. Our biolayer interferometry immunosorbent assay (BLI-ISA) utilizes single-use biosensors in an automated "dip-and-read" format, providing real-time optical measurements of antigen loading, plasma antibody binding, and antibody isotype detection. Complete semi-quantitative results are obtained in less than 20 min. B

SUBMITTER: Dzimianski JV 

PROVIDER: S-EPMC7730435 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature