A real-time PCR assay for rapid identification of inducible and acquired clarithromycin resistance in Mycobacterium abscessus.

ABSTRACT:

Background

Mycobacterium abscessus is a rapidly growing mycobacteria involved in severe infections of the lung, skin, or soft tissue. Macrolides such as clarithromycin are the recommended first line drugs for treatment of M. abscessus infections. However, M. abscessus has dual mechanisms of resistance to macrolides, making treatment by macrolides difficult. A functional erm(41) gene confers for inducible resistance while acquired mutations on the 23S rRNA rrl gene confer for constitutive resistance.

Methods

We have developed a real-time PCR assay to detect both inducible and acquired resistance to clarithromycin, and compared the results to traditional erm(41) and rrl sequencing and phenotypic susceptibility testing using Sensititre™ plates.

Results

Of the total 126 M

SUBMITTER: Sharma MK 

PROVIDER: S-EPMC7730737 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature