Designing next generation recombinant protein expression platforms by modulating the cellular stress response in Escherichia coli.

ABSTRACT:

Background

A cellular stress response (CSR) is triggered upon recombinant protein synthesis which acts as a global feedback regulator of protein expression. To remove this key regulatory bottleneck, we had previously proposed that genes that are up-regulated post induction could be part of the signaling pathways which activate the CSR. Knocking out some of these genes which were non-essential and belonged to the bottom of the E. coli regulatory network had provided higher expression of GFP and L-asparaginase.

Results

We chose the best performing double knockout E. coli BW25113ΔelaAΔcysW and demonstrated its ability to enhance the expression of the toxic Rubella E1 glycoprotein by 2.5-fold by tagging it with sfGFP at the C-terminal end to better quantify expression levels. Tr

SUBMITTER: Guleria R 

PROVIDER: S-EPMC7730785 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature