Label-free optical biomarkers detect early calcific aortic valve disease in a wild-type mouse model.

ABSTRACT:

Background

Calcific aortic valve disease (CAVD) pathophysiology is a complex, multistage process, usually diagnosed at advanced stages after significant anatomical and hemodynamic changes in the valve. Early detection of disease progression is thus pivotal in the development of prevention and mitigation strategies. In this study, we developed a diet-based, non-genetically modified mouse model for early CAVD progression, and explored the utility of two-photon excited fluorescence (TPEF) microscopy for early detection of CAVD progression. TPEF imaging provides label-free, non-invasive, quantitative metrics with the potential to correlate with multiple stages of CAVD pathophysiology including calcium deposition, collagen remodeling and osteogenic differentiation.

Methods

Twenty

SUBMITTER: Tandon I 

PROVIDER: S-EPMC7731510 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature