In vivo assessment of glutamine anaplerosis into the TCA cycle in human pre-malignant and malignant clonal plasma cells.

ABSTRACT:

Background

Overexpression of c-Myc is required for the progression of pre-malignant plasma cells in monoclonal gammopathy of undetermined significance (MGUS) to malignant plasma cells in multiple myeloma (MM). c-Myc also increases glutamine anaplerosis into the tricarboxylic acid (TCA) cycle within cancer cells. Whether increased glutamine anaplerosis is associated with the progression of pre-malignant to malignant plasma cells is unknown.

Methods

Human volunteers (N = 7) and patients with MGUS (N = 11) and MM (N = 12) were prospectively recruited to undergo an intravenous infusion of ¹³C-labeled glutamine followed by a bone marrow aspiration to obtain bone marrow cells and plasma.

Results

Despite notable heterogeneity, stable isotope-resolved metabolomics

SUBMITTER: Gonsalves WI 

PROVIDER: S-EPMC7731537 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature