The effect of vitronectin on the differentiation of embryonic stem cells in a 3D culture system.

ABSTRACT: While stem cell niches in vivo are complex three-dimensional (3D) microenvironments, the relationship between the dimensionality of the niche to its function is unknown. We have created a 3D microenvironment through electrospinning to study the impact of geometry and different extracellular proteins on the development of cardiac progenitor cells (Flk-1(+)) from resident stem cells and their differentiation into functional cardiovascular cells. We have investigated the effect of collagen IV, fibronectin, laminin and vitronectin on the adhesion and proliferation of murine ES cells as well as the effects of these proteins on the number of Flk-1(+) cells cultured in 2D conditions compared to 3D system in a feeder free condition. We found that the number of Flk-1(+) cells was significantly high

SUBMITTER: Heydarkhan-Hagvall S 

PROVIDER: S-EPMC7731733 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature