A reverse transcription loop-mediated isothermal amplification for broad coverage detection of Asian and African Zika virus lineages.

ABSTRACT:

Background

Early detection of Zika virus (ZIKV) infection during the viremia and viruria facilitates proper patient management and mosquito control measurement to prevent disease spread. Therefore, a cost-effective nucleic acid detection method for the diagnosis of ZIKV infection, especially in resource-deficient settings, is highly required.

Methods

In the present study, a single-tube reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the detection of both the Asian and African-lineage ZIKV. The detection limit, strain coverage and cross-reactivity of the ZIKV RT-LAMP assay was evaluated. The sensitivity and specificity of the RT-LAMP were also evaluated using a total of 24 simulated clinical samples. The ZIKV quantitative reverse

SUBMITTER: Teoh BT 

PROVIDER: S-EPMC7731766 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature