Cell-autonomous role of Presenilin in age-dependent survival of cortical interneurons.

ABSTRACT:

Background

Mutations in the PSEN1 and PSEN2 genes are the major cause of familial Alzheimer's disease. Previous studies demonstrated that Presenilin (PS), the catalytic subunit of γ-secretase, is required for survival of excitatory neurons in the cerebral cortex during aging. However, the role of PS in inhibitory interneurons had not been explored.

Methods

To determine PS function in GABAergic neurons, we generated inhibitory neuron-specific PS conditional double knockout (IN-PS cDKO) mice, in which PS is selectively inactivated by Cre recombinase expressed under the control of the endogenous GAD2 promoter. We then performed behavioral, biochemical, and histological analyses to evaluate the consequences of selective PS inactivation in inhibitory neurons.

Results

IN-PS

SUBMITTER: Kang J 

PROVIDER: S-EPMC7731773 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature