Comprehensive analyses of B-cell compartments across the human body reveal novel subsets and a gut-resident memory phenotype.

ABSTRACT: Although human B cells have been extensively studied, most reports have used peripheral blood as a source. Here, we used a unique tissue resource derived from healthy organ donors to deeply characterize human B-cell compartments across multiple tissues and donors. These datasets revealed that B cells in the blood are not in homeostasis with compartments in other tissues. We found striking donor-to-donor variability in the frequencies and isotype of CD27+ memory B cells (MBCs). A comprehensive antibody-based screen revealed markers of MBC and allowed identification of novel MBC subsets with distinct functions defined according to surface expression of CD69 and CD45RB. We defined a tissue-resident MBC phenotype that was predominant in the gut but absent in blood. RNA-sequencing of MBC subset

SUBMITTER: Weisel NM 

PROVIDER: S-EPMC7731793 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature