Comprehensive N- and O-glycosylation mapping of human coagulation factor V.

ABSTRACT:

Background/objective

Coagulation factor V (FV), a multidomain glycoprotein, is an essential cofactor in the blood clotting cascade. FV deficiency is a rare bleeding disorder that results in poor clotting after an injury or surgery. The only treatment for the disease is infusions of fresh frozen plasma and blood platelets. Glycosylation affects the biological activity, pharmacokinetics, immunogenicity, and in vivo clearance rate of proteins in the plasma. The glycan profile of FV, as well as how it affects the activity, stability, and immunogenicity, remains unknown.

Methods

In this study, we comprehensively mapped the glycosylation patterns of human plasma-derived FV by combining multienzyme digestion, hydrophilic interaction chromatography enrichment of glycopeptides, and a

SUBMITTER: Ma C 

PROVIDER: S-EPMC7732234 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature