Surprisingly Fast Interface and Elbow Angle Dynamics of Antigen-Binding Fragments.

ABSTRACT: Fab consist of a heavy and light chain and can be subdivided into a variable (V _H and V _L ) and a constant region (C _H 1 and C _L ). The variable region contains the complementarity-determining region (CDR), which is formed by six hypervariable loops, shaping the antigen binding site, the paratope. Apart from the CDR loops, both the elbow angle and the relative interdomain orientations of the V _H -V _L and the C _H 1-C _L domains influence the shape of the paratope. Thus, characterization of the interface and elbow angle dynamics is essential to antigen specificity. We studied nine antigen-binding fragments (Fab) to investigate the influence of affinit

SUBMITTER: Fernandez-Quintero ML 

PROVIDER: S-EPMC7732698 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature