Extracting Transition Rates in Particle Tracking Using Analytical Diffusion Distribution Analysis.

ABSTRACT: Single-particle tracking is an important technique in the life sciences to understand the kinetics of biomolecules. The analysis of apparent diffusion coefficients in vivo, for example, enables researchers to determine whether biomolecules are moving alone, as part of a larger complex, or are bound to large cellular components such as the membrane or chromosomal DNA. A remaining challenge has been to retrieve quantitative kinetic models, especially for molecules that rapidly switch between different diffusional states. Here, we present analytical diffusion distribution analysis (anaDDA), a framework that allows for extracting transition rates from distributions of apparent diffusion coefficients calculated from short trajectories that feature less than 10 localizations per track. Under the

SUBMITTER: Vink JNA 

PROVIDER: S-EPMC7732728 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature