The Relative Densities of Cytoplasm and Nuclear Compartments Are Robust against Strong Perturbation.

ABSTRACT: The cell nucleus is a compartment in which essential processes such as gene transcription and DNA replication occur. Although the large amount of chromatin confined in the finite nuclear space could install the picture of a particularly dense organelle surrounded by less dense cytoplasm, recent studies have begun to report the opposite. However, the generality of this newly emerging, opposite picture has so far not been tested. Here, we used combined optical diffraction tomography and epi-fluorescence microscopy to systematically quantify the mass densities of cytoplasm, nucleoplasm, and nucleoli of human cell lines, challenged by various perturbations. We found that the nucleoplasm maintains a lower mass density than cytoplasm during cell cycle progression by scaling its volume to match t

SUBMITTER: Kim K 

PROVIDER: S-EPMC7732746 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature