Infrared Excitation Induces Heating and Calcium Microdomain Hyperactivity in Cortical Astrocytes.

ABSTRACT: Unraveling how neural networks process and represent sensory information and how these cellular signals instruct behavioral output is a main goal in neuroscience. Two-photon activation of optogenetic actuators and calcium (Ca²⁺) imaging with genetically encoded indicators allow, respectively, the all-optical stimulation and readout of activity from genetically identified cell populations. However, these techniques locally expose the brain to high near-infrared light doses, raising the concern of light-induced adverse effects on the biology under study. Combining 2P imaging of Ca²⁺ transients in GCaMP6f-expressing cortical astrocytes and unbiased machine-based event detection, we demonstrate the subtle build-up of aberrant microdomain Ca²⁺ transients in the

SUBMITTER: Schmidt E 

PROVIDER: S-EPMC7732816 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature