High repetitive arginine in the anterior of PCV3 capsid protein is a severe obstacle for its expression in E. coli.
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SUBMITTER: Liu BY
PROVIDER: S-EPMC7732928 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
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High repetitive arginine in the anterior of PCV3 capsid protein is a severe obstacle for its expression in E. coli.
Liu Bing Yan BY Gao Bin B Liu Meng Zhi MZ Zhang Ting Ting TT Liu Bao Shan BS Chen Ze Liang ZL
AMB Express 20201211 1
Porcine circovirus type 3 (PCV3) is a novel circovirus identified in sows with PDNS-like clinical signs and reproductive failure. The capsid protein (CAP) of PCV3 is expected to be an effective vaccine candidate. Here, we expressed the original capsid protein, truncated capsid protein without anterior highly repetitive arginine (ΔCAP) and their codon-optimized counterparts in E. coli. These results showed that lots of repeated arginine could severely lower the expression of PCV3 capsid protein i ...[more]
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