A P2rx7 Passenger Mutation Affects the Vitality and Function of T cells in Congenic Mice.

ABSTRACT: Among laboratory mouse strains many genes are differentially expressed in the same cell population. As consequence, gene targeting in 129-derived embryonic stem cells (ESCs) and backcrossing the modified mice onto the C57BL/6 background can introduce passenger mutations in the close proximity of the targeted gene. Here, we demonstrate that several transgenic mice carry a P2rx7 passenger mutation that affects the function of T cells. By the example of P2rx4^tm1Rass we demonstrate that P2X4ko T cells express higher levels of P2X7 and are more sensitive toward the P2X7 activators ATP and NAD⁺, rendering these cells more vulnerable toward NAD-induced cell death (NICD) compared with wild type (WT). The enhanced NICD sensitivity confounded functional assays e.g. cytok

SUBMITTER: Er-Lukowiak M 

PROVIDER: S-EPMC7733020 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature