Single-nucleus RNA-seq identifies transcriptional heterogeneity in multinucleated skeletal myofibers.

ABSTRACT: While the majority of cells contain a single nucleus, cell types such as trophoblasts, osteoclasts, and skeletal myofibers require multinucleation. One advantage of multinucleation can be the assignment of distinct functions to different nuclei, but comprehensive interrogation of transcriptional heterogeneity within multinucleated tissues has been challenging due to the presence of a shared cytoplasm. Here, we utilized single-nucleus RNA-sequencing (snRNA-seq) to determine the extent of transcriptional diversity within multinucleated skeletal myofibers. Nuclei from mouse skeletal muscle were profiled across the lifespan, which revealed the presence of distinct myonuclear populations emerging in postnatal development as well as aging muscle. Our datasets also provided a platform for discove

SUBMITTER: Petrany MJ 

PROVIDER: S-EPMC7733460 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature