Chemotherapy sensitivity testing on ovarian cancer cells isolated from malignant ascites.

ABSTRACT:

Background

In epithelial ovarian cancer (EOC), 15-20% of the tumors do not respond to first-line chemotherapy (paclitaxel with platinum-based therapy), and in recurrences this number increases. Our aim is to determine the feasibility of cell proliferation assays of tumor cells isolated from malignant ascites to predict in vitro chemotherapy sensitivity, and to correlate these results with clinical outcome.

Materials and methods

Ascites was collected from twenty women with advanced EOC. Cell samples were enriched for tumor cells and EOC origin was confirmed by intracellular staining of CK7, surface staining of CA125 and EpCAM, and HE4 gene expression. In vitro sensitivity to chemotherapy was determined in cell proliferation assays using intracellular ATP

SUBMITTER: den Ouden JE 

PROVIDER: S-EPMC7733621 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature