The SERCA residue Glu340 mediates interdomain communication that guides Ca²⁺ transport.

ABSTRACT: The sarco(endo)plasmic reticulum Ca²⁺-ATPase (SERCA) is a P-type ATPase that transports Ca²⁺ from the cytosol into the sarco(endo)plasmic reticulum (SR/ER) lumen, driven by ATP. This primary transport activity depends on tight coupling between movements of the transmembrane helices forming the two Ca²⁺-binding sites and the cytosolic headpiece mediating ATP hydrolysis. We have addressed the molecular basis for this intramolecular communication by analyzing the structure and functional properties of the SERCA mutant E340A. The mutated Glu340 residue is strictly conserved among the P-type ATPase family of membrane transporters and is located at a seemingly strategic position at the interface between the phosphorylation domain and the cytosolic ends of 5 of SE

SUBMITTER: Geurts MMG 

PROVIDER: S-EPMC7733806 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature