Structure of the Dietzia Mrp complex reveals molecular mechanism of this giant bacterial sodium proton pump.

ABSTRACT: Multiple resistance and pH adaptation (Mrp) complexes are sophisticated cation/proton exchangers found in a vast variety of alkaliphilic and/or halophilic microorganisms, and are critical for their survival in highly challenging environments. This family of antiporters is likely to represent the ancestor of cation pumps found in many redox-driven transporter complexes, including the complex I of the respiratory chain. Here, we present the three-dimensional structure of the Mrp complex from a Dietzia sp. strain solved at 3.0-Å resolution using the single-particle cryoelectron microscopy method. Our structure-based mutagenesis and functional analyses suggest that the substrate translocation pathways for the driving substance protons and the substrate sodium ions are separated in two m

SUBMITTER: Li B 

PROVIDER: S-EPMC7733839 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature