Detection and elimination of a novel non-toxigenic Clostridioides difficile strain from the microbiota of a mouse colony.

ABSTRACT: Clostridioides difficile is an enteric bacterial pathogen that can a cause nosocomial infection leading to debilitating colitis. The development of a murine model of C. difficile infection has led to fundamental discoveries in disease pathogenesis and the host immune response to infection. Recently, C. difficile endogenously present in the microbiota of mice has been reported and was found to complicate interpretation of mouse studies. Here, we report a novel C. difficile strain, named NTCD-035, isolated from the microbiota of our mouse colony. The presence of NTCD-035 in mice prior to challenge with a highly pathogenic C. difficile strain (VPI10463) led to significantly reduced disease severity. Phylogenetic characterization derived from whole genome seq

SUBMITTER: Maslanka JR 

PROVIDER: S-EPMC7734020 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature