Small subset of Wnt-activated cells is an initiator of regrowth in colorectal cancer organoids after irradiation.

ABSTRACT: Most colorectal cancers (CRCs) are differentiated adenocarcinomas, which maintain expression of both stemness and differentiation markers. This observation suggests that CRC cells could retain a regeneration system of normal cells upon injury. However, the role of stemness in cancer cell regeneration after irradiation is poorly understood. Here, we examined the effect of radiation on growth, stemness, and differentiation in organoids derived from differentiated adenocarcinomas. Following a sublethal dose of irradiation, proliferation and stemness markers, including Wnt target genes, were drastically reduced, but differentiation markers remained. After a static growth phase after high dose of radiation, regrowth foci appeared; these consisted of highly proliferating cells that expressed ste

SUBMITTER: Endo H 

PROVIDER: S-EPMC7734167 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature