Minimizing scattering-induced phase errors in differential interference contrast microscopy.

ABSTRACT:

Significance

Differential interference contrast (DIC) microscopes allow noninvasive in vivo observation of transparent microstructures in tissue without the use of fluorescent dyes or genetic modification. We show how to modify a DIC microscope to measure the sample phase distribution accurately and in real-time even deep inside sample tissue.

Aim

Our aim is to improve the DIC microscope's phase measurement to remove the phase bias that occurs in the presence of strong scattering.

Approach

A quarter-wave plate was added in front of the polarization camera, allowing a modified phase calculation to incorporate all four polarization orientation angles (0 deg, 45 deg, 90 deg, and 135 deg) captured simultaneously by the polarization camera, followed by deconvolution.

Re

SUBMITTER: Takano W 

PROVIDER: S-EPMC7734411 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature