A novel labeling strategy reveals that myosin Va and myosin Vb bind the same dendritically polarized vesicle population.

ABSTRACT: Neurons are specialized cells with a polarized geometry and several distinct subdomains that require specific complements of proteins. Delivery of transmembrane proteins requires vesicle transport, which is mediated by molecular motor proteins. The myosin V family of motor proteins mediates transport to the barbed end of actin filaments, and little is known about the vesicles bound by myosin V in neurons. We developed a novel strategy to visualize myosin V-labeled vesicles in cultured hippocampal neurons and systematically characterized the vesicle populations labeled by myosin Va and Vb. We find that both myosins bind vesicles that are polarized to the somatodendritic domain where they undergo bidirectional long-range transport. A series of two-color imaging experiments showed that myosin

SUBMITTER: Frank M 

PROVIDER: S-EPMC7734653 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature