Rck of Salmonella Typhimurium Delays the Host Cell Cycle to Facilitate Bacterial Invasion.

ABSTRACT: Salmonella Typhimurium expresses on its outer membrane the protein Rck which interacts with the epidermal growth factor receptor (EGFR) of the plasma membrane of the targeted host cells. This interaction activates signaling pathways, leading to the internalization of Salmonella. Since EGFR plays a key role in cell proliferation, we sought to determine the influence of Rck mediated infection on the host cell cycle. By analyzing the DNA content of uninfected and infected cells using flow cytometry, we showed that the Rck-mediated infection induced a delay in the S-phase (DNA replication phase) of the host cell cycle, independently of bacterial internalization. We also established that this Rck-dependent delay in cell cycle progression was accompanied by an increased level of ho

SUBMITTER: Mambu J 

PROVIDER: S-EPMC7734966 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature