Combinatorial nanodot stripe assay to systematically study cell haptotaxis.

ABSTRACT: Haptotaxis is critical to cell guidance and development and has been studied in vitro using either gradients or stripe assays that present a binary choice between full and zero coverage of a protein cue. However, stripes offer only a choice between extremes, while for gradients, cell receptor saturation, migration history, and directional persistence confound the interpretation of cellular responses. Here, we introduce nanodot stripe assays (NSAs) formed by adjacent stripes of nanodot arrays with different surface coverage. Twenty-one pairwise combinations were designed using 0, 1, 3, 10, 30, 44 and 100% stripes and were patterned with 200 × 200, 400 × 400 or 800 × 800 nm² nanodots. We studied the migration choices of C2C12 myoblasts that express neogenin on NSAs (and three-step

SUBMITTER: Dlamini M 

PROVIDER: S-EPMC7735170 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature