Single-Peptide TR-FRET Detection Platform for Cysteine-Specific Post-Translational Modifications.

ABSTRACT: Post-translational modifications (PTMs) are one of the most important regulatory mechanisms in cells, and they play key roles in cell signaling both in health and disease. PTM catalyzing enzymes have become significant drug targets, and therefore, tremendous interest has been focused on the development of broad-scale assays to monitor several different PTMs with a single detection platform. Most of the current methodologies suffer from low throughput or rely on antibody recognition, increasing the assay costs, and decreasing the multifunctionality of the assay. Thus, we have developed a sensitive time-resolved Förster resonance energy transfer (TR-FRET) detection method for PTMs of cysteine residues using a single-peptide approach performed in a 384-well format. In the developed assay, the

SUBMITTER: Eskonen V 

PROVIDER: S-EPMC7735653 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature