Chemistry of Autumn Colors: Quantitative Spectrophotometric Analysis of Anthocyanins and Carotenoids and Qualitative Analysis of Anthocyanins by Ultra-performance Liquid Chromatography-Tandem Mass Spectrometry.

ABSTRACT: The autumnal leaf color change is a familiar phenomenon to most students living in temperate climate zones. The extraction and analysis of the pigments of the autumn leaves provide an engaging way to study the phenomenon utilizing both simple as well as more sophisticated analytical methods. In this laboratory experiment, students extract the red and yellow pigments, that is, anthocyanins and carotenoids from leaves and separate them from each other by liquid-liquid extractions. From the separated phases, anthocyanin and carotenoid concentrations can be evaluated visually or spectrophotometrically. The anthocyanins are analyzed by a rapid and simple ultra-performance liquid chromatography-tandem mass spectrometry method to discover which of the six most common groups of anthocyanins are pr

SUBMITTER: Manninen M 

PROVIDER: S-EPMC7735701 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature