[⁸⁹Zr]Zr-DBN labeled cardiopoietic stem cells proficient for heart failure.

ABSTRACT:

Introduction

Radiolabeling of stem cells with a positron emitting radioisotope represents a major advancement in regenerative biotherapy enabling non-invasive imaging. To assess the value of such an approach in a clinically relevant scenario, the tolerability and therapeutic aptitude of [⁸⁹Zr]zirconium-p-isothiocyanatobenzyl-desferrioxamine ([⁸⁹Zr]Zr-DBN) labeled human cardiopoietic stem cells (CPs) were evaluated in a model of ischemic heart failure.

Methods and results

[⁸⁹Zr]Zr-DBN based radiolabeling of human CPs yielded [⁸⁹Zr]Zr-DBN-CPs with radioactivity yield of 0.70 ± 0.20 MBq/10⁶ cells and excellent label stability. Compared to unlabeled cell counterparts, [⁸⁹Zr]Zr-DBN-CPs maintained morphology, viabil

SUBMITTER: Bansal A 

PROVIDER: S-EPMC7736260 | biostudies-literature | 2020 Nov - Dec

REPOSITORIES: biostudies-literature