Differential Induction of the ADAM17 Regulators iRhom1 and 2 in Endothelial Cells.

ABSTRACT: Background: Endothelial function significantly depends on the proteolytic release of surface expressed signal molecules, their receptors and adhesion molecules via the metalloproteinase ADAM17. The pseudoproteases iRhom1 and 2 independently function as adapter proteins for ADAM17 and are essential for the maturation, trafficking, and activity regulation of ADAM17. Bioinformatic data confirmed that immune cells predominantly express iRhom2 while endothelial cells preferentially express iRhom1. Objective: Here, we investigate possible reasons for higher iRhom1 expression and potential inflammatory regulation of iRhom2 in endothelial cells and analyze the consequences for ADAM17 maturation and function. Methods: Primary endothelial cells were cultured in absence and prese

SUBMITTER: Babendreyer A 

PROVIDER: S-EPMC7736406 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature