P2Y₂ receptors mediate nucleotide-induced EGFR phosphorylation and stimulate proliferation and tumorigenesis of head and neck squamous cell carcinoma cell lines.

ABSTRACT:

Objectives

To assess functional expression of the P2Y₂ nucleotide receptor (P2Y₂R) in head and neck squamous cell carcinoma (HNSCC) cell lines and define its role in nucleotide-induced epidermal growth factor receptor (EGFR) transactivation. The use of anti-EGFR therapeutics to treat HNSCC is hindered by intrinsic and acquired drug resistance. Defining novel pathways that modulate EGFR signaling could identify additional targets to treat HNSCC.

Materials and methods

In human HNSCC cell lines CAL27 and FaDu and the mouse oral cancer cell line MOC2, P2Y₂R contributions to extracellular nucleotide-induced changes in intracellular free Ca²⁺ concentration and EGFR and extracellular signal-regulated kinase (ERK1/2) phosphorylation were d

SUBMITTER: Woods LT 

PROVIDER: S-EPMC7736485 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature