Characterization of primary mouse hepatocyte spheroids as a model system to support investigations of drug-induced liver injury.

ABSTRACT: Primary mouse hepatocytes isolated from genetically defined and/or diverse lines and disease models are a valuable resource for studying the impact of genetic and environmental factors on drug response and disease. However, standard monolayer cultures result in a rapid decline in mouse hepatocyte viability and functionality. Therefore, we evaluated 3D spheroid methodology for long-term culture of primary mouse hepatocytes, initially to support investigations of drug-induced liver injury (DILI). Primary hepatocytes isolated from male and female C57BL/6J mice were used to generate spheroids by spontaneous self-aggregation in ultra-low attachment plates. Spheroids with well-defined perimeters were observed within 5 days after seeding and retained morphology, ATP, and albumin levels for an add

SUBMITTER: Nautiyal M 

PROVIDER: S-EPMC7736539 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature