Ca²⁺ Dependence of Volume-Regulated VRAC/LRRC8 and TMEM16A Cl^- Channels.

ABSTRACT: All vertebrate cells activate Cl^- currents (I_{Cl ,swell}) when swollen by hypotonic bath solution. The volume-regulated anion channel VRAC has now been identified as LRRC8/SWELL1. However, apart from VRAC, the Ca²⁺-activated Cl^- channel (CaCC) TMEM16A and the phospholipid scramblase and ion channel TMEM16F were suggested to contribute to cell swelling-activated whole-cell currents. Cell swelling was shown to induce Ca²⁺ release from the endoplasmic reticulum and to cause subsequent Ca²⁺ influx. It is suggested that TMEM16A/F support intracellular Ca²⁺ signaling and thus Ca²⁺-dependent activation of VRAC. In the present study, we tried to clarify the contribution of TMEM16A to I_Cl

SUBMITTER: Centeio R 

PROVIDER: S-EPMC7736618 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature