Single-molecule DNA sequencing of widely varying GC-content using nucleotide release, capture and detection in microdroplets.

ABSTRACT: Despite remarkable progress in DNA sequencing technologies there remains a trade-off between short-read platforms, having limited ability to sequence homopolymers, repeated motifs or long-range structural variation, and long-read platforms, which tend to have lower accuracy and/or throughput. Moreover, current methods do not allow direct readout of epigenetic modifications from a single read. With the aim of addressing these limitations, we have developed an optical electrowetting sequencing platform that uses step-wise nucleotide triphosphate (dNTP) release, capture and detection in microdroplets from single DNA molecules. Each microdroplet serves as a reaction vessel that identifies an individual dNTP based on a robust fluorescence signal, with the detection chemistry extended to enable

SUBMITTER: Puchtler TJ 

PROVIDER: S-EPMC7736801 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature