Comparison of the two up-to-date sequencing technologies for genome assembly: HiFi reads of Pacific Biosciences Sequel II system and ultralong reads of Oxford Nanopore.

ABSTRACT:

Background

The availability of reference genomes has revolutionized the study of biology. Multiple competing technologies have been developed to improve the quality and robustness of genome assemblies during the past decade. The 2 widely used long-read sequencing providers-Pacific Biosciences (PacBio) and Oxford Nanopore Technologies (ONT)-have recently updated their platforms: PacBio enables high-throughput HiFi reads with base-level resolution of >99%, and ONT generated reads as long as 2 Mb. We applied the 2 up-to-date platforms to a single rice individual and then compared the 2 assemblies to investigate the advantages and limitations of each.

Results

The results showed that ONT ultralong reads delivered higher contiguity, producing a total of 18 contigs of which 10 were

SUBMITTER: Lang D 

PROVIDER: S-EPMC7736813 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature