Systematic prediction of genes functionally associated with bacterial retrons and classification of the encoded tripartite systems.

ABSTRACT: Bacterial retrons consist of a reverse transcriptase (RT) and a contiguous non-coding RNA (ncRNA) gene. One third of annotated retrons carry additional open reading frames (ORFs), the contribution and significance of which in retron biology remains to be determined. In this study we developed a computational pipeline for the systematic prediction of genes specifically associated with retron RTs based on a previously reported large dataset representative of the diversity of prokaryotic RTs. We found that retrons generally comprise a tripartite system composed of the ncRNA, the RT and an additional protein or RT-fused domain with diverse enzymatic functions. These retron systems are highly modular, and their components have coevolved to different extents. Based on the additional module, we c

SUBMITTER: Mestre MR 

PROVIDER: S-EPMC7736814 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature