Bacterial tRNA 2'-O-methylation is dynamically regulated under stress conditions and modulates innate immune response.

ABSTRACT: RNA modifications are a well-recognized way of gene expression regulation at the post-transcriptional level. Despite the importance of this level of regulation, current knowledge on modulation of tRNA modification status in response to stress conditions is far from being complete. While it is widely accepted that tRNA modifications are rather dynamic, such variations are mostly assessed in terms of total tRNA, with only a few instances where changes could be traced to single isoacceptor species. Using Escherichia coli as a model system, we explored stress-induced modulation of 2'-O-methylations in tRNAs by RiboMethSeq. This analysis and orthogonal analytical measurements by LC-MS show substantial, but not uniform, increase of the Gm18 level in selected tRNAs under mild bacteriostatic antib

SUBMITTER: Galvanin A 

PROVIDER: S-EPMC7736821 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature