PLEKHG5 regulates autophagy, survival and MGMT expression in U251-MG glioblastoma cells.

ABSTRACT: A signalling pathway involving PLEKHG5 (guanine exchange factor) for the Ras superfamily member RAB26 to transcription factor NF-κB was discovered in autophagy. PLEKHG5 was reported in glioblastoma multiforme (GBM) and correlates with patient survival. Thus, the generation of a cellular model for understanding PLEKHG5 signalling is the study purpose. We generated a CRISPR/Cas9-mediated knockout of PLEKHG5 in U251-MG glioblastoma cells and analysed resulting changes. Next, we used a mRFP-GFP-LC3⁺ reporter for visualisation of autophagic defects and rescued the phenotype of PLEKHG5 wildtype via transduction of a constitutively active RAB26QL-plasmid. Effects of overexpressing RAB26 were investigated and correlated with the O⁶-methylguanine-DNA methyltransferase (MGMT) a

SUBMITTER: Witte KE 

PROVIDER: S-EPMC7736842 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature