BRAF^V600E mutation impinges on gut microbial markers defining novel biomarkers for serrated colorectal cancer effective therapies.

ABSTRACT:

Background

Colorectal cancer (CRC) harboring BRAF^V600E mutation exhibits low response to conventional therapy and poorest prognosis. Due to the emerging correlation between gut microbiota and CRC carcinogenesis, we investigated in serrated BRAF^V600E cases the existence of a peculiar fecal microbial fingerprint and specific bacterial markers, which might represent a tool for the development of more effective clinical strategies.

Methods

By injecting human CRC stem-like cells isolated from BRAF^V600E patients in immunocompromised mice, we described a new xenogeneic model of this subtype of CRC. By performing bacterial 16S rRNA sequencing, the fecal microbiota profile was then investigated either in CRC-carrying mice or in a cohort of human CRC s

SUBMITTER: Trivieri N 

PROVIDER: S-EPMC7737386 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature