The Genome Segments of Bluetongue Virus Differ in Copy Number in a Host-Specific Manner.

ABSTRACT: Genome segmentation is mainly thought to facilitate reassortment. Here, we show that segmentation can also allow differences in segment abundance in populations of bluetongue virus (BTV). BTV has a genome consisting in 10 segments, and its cycle primarily involves periodic alternation between ruminants and Culicoides biting midges. We have developed a reverse transcription-quantitative PCR (RT-qPCR) approach to quantify each segment in wild BTV populations sampled in both ruminants and midges during an epizootic. Segment frequencies deviated from equimolarity in all hosts. Interestingly, segment frequencies were reproducible and distinct between ruminants and biting midges. Beyond a putative regulatory role in virus expression, this phenomenon could lead to different evolution rates betwee

SUBMITTER: Moreau Y 

PROVIDER: S-EPMC7737730 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature