The Role of CCL20-CCR6 Axis in Ovarian Cancer Metastasis.

ABSTRACT:

Background

Chemokine networks play a key and complex role in tumor progression. CCL20 and its unique receptor CCR6 have been reported to mediate malignant biological activities in various cancers, but their role in ovarian cancer metastasis remains unclear.

Purpose

Our study aims to explore the effect of CCL20-CCR6 axis on ovarian cancer metastasis and its potential mechanism.

Methods

The transwell assay was used to detect the cell migration and invasion after CCL20 treatment. The CCK-8 assay was used to detect the cell viability after CCL20 treatment and CCR6 depletion. The mRNA and protein expression were assayed through qRT-PCR and Western blotting. The siRNAs and CRISPR-Cas9 system were adopted to suppress CCR6 expression. Intraperitoneal xenograft mouse model was

SUBMITTER: Liu W 

PROVIDER: S-EPMC7738160 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature