Quantitative dissection of transcription in development yields evidence for transcription-factor-driven chromatin accessibility.

ABSTRACT: Thermodynamic models of gene regulation can predict transcriptional regulation in bacteria, but in eukaryotes, chromatin accessibility and energy expenditure may call for a different framework. Here, we systematically tested the predictive power of models of DNA accessibility based on the Monod-Wyman-Changeux (MWC) model of allostery, which posits that chromatin fluctuates between accessible and inaccessible states. We dissected the regulatory dynamics of hunchback by the activator Bicoid and the pioneer-like transcription factor Zelda in living Drosophila embryos and showed that no thermodynamic or non-equilibrium MWC model can recapitulate hunchback transcription. Therefore, we explored a model where DNA accessibility is not the result of thermal fluctuations but is

SUBMITTER: Eck E 

PROVIDER: S-EPMC7738189 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature