Use of in vitro assays to identify antibiotics that are cytotoxic to normal equine chondrocytes and synovial cells.

ABSTRACT:

Background

Intra-articular (IA) antibiotic usage is prevalent in equine practice. However, recent emergence of antimicrobial resistance prompts re-evaluation of antibiotic selection, particularly when used prophylactically. Furthermore, many commonly used antibiotics exert direct cytotoxicity to equine cells, and appropriate IA doses have not been defined.

Objectives

To screen antibiotics in vitro as an initial assessment of cytotoxicity against normal equine joint cells in monolayer culture and explant tissues.

Study design

In vitro experimental study.

Methods

Chondrocytes and synovial cells were harvested from three horses and plated on 24-well plates (100 000 cells/wells in triplicate) for 48 hours prior to addition of antibiotics. Joint cells were exposed t

SUBMITTER: Pezzanite L 

PROVIDER: S-EPMC7738387 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature