Development of Fluorescence Polarization Immunoassay for Imidacloprid in Environmental and Agricultural Samples.

ABSTRACT: A fluorescence polarization immunoassay (FPIA) for the determination of imidacloprid (IMI) was developed with advantages of simple operation and short assay time. The haptens of IMI, acetamiprid (ACE), and thiamethoxam (THI) were conjugated with fluorescein isothiocyanate ethylenediamine (EDF) and 4'-Aminomethyl fluorescein (AMF), respectively, to prepare six fluorescence tracers. The conjugation of IMI hapten and EDF (IMI-EDF) was selected to develop the FPIA due to the largest fluorescent polarization value increase in the presence of anti-IMI monoclonal antibody. Under the optimum condition, the limit of detection, 50% inhibition concentration and detection range of the FPIA were 1.7, 4.8, and 1.7-16.3 μg/L, respectively. The cross-reactivities (CRs) with the analogs of IMI were negligi

SUBMITTER: Zhou L 

PROVIDER: S-EPMC7738439 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature