Molecular stratification by BCL2A1 and AIM2 provides additional prognostic value in penile squamous cell carcinoma.

ABSTRACT: Background: Lymph node metastasis is the most unfavorable prognostic factor of penile squamous cell carcinoma (PSCC). However, patients with the same lymph node status have different outcomes, and molecular classifiers for precise prognostic assessments are lacking. Methods: Comprehensive genomic profiling and high-content proliferation screening were performed in eight PSCC and normal tissue pairs and in cell lines. BCL2A1 and AIM2 were selected and further evaluated by qPCR and Western blot. The clinical relevance and prognostic value of the target genes were validated via immunohistochemistry in a cohort of 220 PSCC patients with a defined pN stage. Finally, the biological functions and molecular mechanisms of BCL2A1 and AIM2 were investigated in vitro and

SUBMITTER: Tan X 

PROVIDER: S-EPMC7738875 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature