Navitoclax combined with Alpelisib effectively inhibits Merkel cell carcinoma cell growth in vitro.

ABSTRACT:

Background

Merkel cell carcinoma (MCC) is a highly malignant skin cancer. Despite major treatment improvements during the last decade, up to 50% of patients do not respond to therapy or develop recurrent disease. For these patients, alternative treatment options are urgently needed. Here, we assessed the efficacy of the combination of the BCL-2 inhibitor Navitoclax and the PI3K p110α inhibitor Alpelisib in MCC cell lines.

Methods

The expression of BCL-2 was assessed by immunohistochemistry in MCC and MCC cell lines. Treatment with Navitoclax and Alpelisib alone and in combination was performed on four MCC cell lines. The decrease of cell viability during treatment was assessed by XTT assay and visualized for the combinations by 3D combinatorial index plotting. The increase o

SUBMITTER: Chteinberg E 

PROVIDER: S-EPMC7739210 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature