Re-annotation of genome microbial coding-sequences: finding new genes and inaccurately annotated genes.

ABSTRACT:

Background

Analysis of any newly sequenced bacterial genome starts with the identification of protein-coding genes. Despite the accumulation of multiple complete genome sequences, which provide useful comparisons with close relatives among other organisms during the annotation process, accurate gene prediction remains quite difficult. A major reason for this situation is that genes are tightly packed in prokaryotes, resulting in frequent overlap. Thus, detection of translation initiation sites and/or selection of the correct coding regions remain difficult unless appropriate biological knowledge (about the structure of a gene) is imbedded in the approach.

Results

We have developed a new program that automatically identifies biologically significant candidate genes in a bacte

SUBMITTER: Bocs S 

PROVIDER: S-EPMC77393 | biostudies-literature | 2002

REPOSITORIES: biostudies-literature