Lipid and protein corona of food-grade TiO₂ nanoparticles in simulated gastrointestinal digestion.

ABSTRACT: In the presence of biological matrices, engineered nanomaterials, such as TiO₂, develop a biomolecular corona composed of lipids, proteins, etc. In this study, we analyzed the biocorona formed on the food grade TiO₂ (E171) going through an in vitro simulated gastrointestinal digestion system in either a fasting food model (FFM), a standardized food model (SFM), or a high fat food model (HFFM). Lipids and proteins were extracted from the biocorona and underwent untargeted lipidomic and label-free shotgun proteomic analyses. Our results showed that the biocorona composition was different before and after food digestion. After digestion, more diverse lipids were adsorbed compared to proteins, most of which were the enzymes added to the simulated digestion system.

SUBMITTER: Coreas R 

PROVIDER: S-EPMC7742882 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature