The development of a novel diagnostic PCR for Madurella mycetomatis using a comparative genome approach.

ABSTRACT:

Background

Eumycetoma is a neglected tropical disease most commonly caused by the fungus Madurella mycetomatis. Identification of eumycetoma causative agents can only be reliably performed by molecular identification, most commonly by species-specific PCR. The current M. mycetomatis specific PCR primers were recently discovered to cross-react with Madurella pseudomycetomatis. Here, we used a comparative genome approach to develop a new M. mycetomatis specific PCR for species identification.

Methodology

Predicted-protein coding sequences unique to M. mycetomatis were first identified in BLASTCLUST based on E-value, size and presence of orthologues. Primers were then developed for 16 unique sequences and evaluated against 60 M. mycetomatis isolates and other eumycetoma causing

SUBMITTER: Lim W 

PROVIDER: S-EPMC7743967 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature