Gain-of-function hot spot mutant p53^R248Q regulation of integrin/FAK/ERK signaling in esophageal squamous cell carcinoma.

ABSTRACT:

Purpose

TP53, encoding the protein p53, is among the most frequently mutated genes in all cancers. A high frequency of 60 - 90% mutations is seen in esophageal squamous cell carcinoma (ESCC) patients. Certain p53 mutants show gain-of-function (GoF) oncogenic features unrelated to its wild type functions.

Methods

This study functionally characterized a panel of p53 mutants in individual ESCC cell lines and assayed for GoF oncogenic properties.

Results

The ESCC cell line with endogenous p53^R248Q expression showed suppressed tumor growth in an immunocompromised mouse model and suppressed colony growth in in vitro three-dimensional culture, when depleted of the endogenous p53 protein expression. This suppression is accompanied by suppressed cell cycle progressi

SUBMITTER: Yu VZ 

PROVIDER: S-EPMC7744772 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature